Open AccessSelective Fermentation and Prebiotic Index of Sago (Metroxylon sagu) Resistant Starches Type III by Rat Fecal Cultures
Author(s) -
Tan Zi-Ni,
Rizwan Ahmad
Publication year - 2020
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/716/1/012002
Subject(s) - prebiotic , inulin , fermentation , food science , chemistry , resistant starch , biology , starch
One of the criteria for prebiotic substance is its selective fermentation by beneficial gut microbes. Previously, sago resistant starch type III (RS 3 ) produced from Metroxylon sagu starch had been proven to resist digestion by gastrointestinal acidity and enzymes. In this research, sago RS 3 was evaluated for its selective fermentation and prebiotic potential by fecal cultures. Sago RS 3 was incubated with fecal microbial cultures for 72 hours at 37°C and samples were measured for microbial growth, prebiotic index, organic acid concentration and β-glucuronidase activity during the fermentation. Commercial prebiotics FOS (fructooligosaccharides) and inulin were used as comparison. Sago RS 3 was selectively fermented by fecal beneficial lactobacilli and bifidobacteria while decreasing the growth of detrimental bacteroides, clostridia and enterobacteria. The prebiotic index of sago RS 3 , FOS and inulin was +12.19, +9.45 and +6.82, respectively. The butyric acid molar concentration in media with sago RS 3 was comparable with FOS and inulin, and low activity of β-glucuronidase was detected in medium with sago RS 3 . Sago RS 3 exhibited prebiotic characteristics comparable with commercial prebiotics and their potential prebiotic function is worth for further in vivo assessment.