Open AccessBiocompatibility Study of Ti-based Alloys Fabricated by Spark Plasma Sintering
Author(s) -
M. R. Mahundla,
Wallace Matizamhuka,
Ronald Machaka,
Atsusi Yamamoto,
Mxolisi Brendon Shongwe
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/655/1/012021
Subject(s) - biocompatibility , materials science , spark plasma sintering , contact angle , protein adsorption , titanium , wetting , alloy , titanium alloy , adsorption , metallurgy , anatase , nuclear chemistry , chemical engineering , composite material , ceramic , chemistry , organic chemistry , engineering , polymer , biochemistry , catalysis , photocatalysis
Titanium (Ti) alloys often find their way in orthopaedic applications owing to their superior mechanical properties, biocompatibility, corrosion resistance and low Young’s modulus. Nano/sub-micron grain structured commercially pure titanium (CP Ti), Ti-6Al-4V (Ti-64) and Ti-34Nb-25Zr (TNZ) alloys were fabricated via mechanical alloying (MA) of elemental powders followed by spark-plasma sintering (SPS). The powders were milled at 500rpm for 5h and sintered at a temperature of 1200°C, heating rate of 100°C/min, dwelling time of 10min and a pressure of 50MPa. The sintered samples were metallographically prepared to a mirror finish surface in 5:2 ratio of colloidal silica and hydrogen peroxide (H2O2) mixture. Biocompatibility tests were conducted by water soluble tetrazolium and 1-methoxy phenazinium methylsufate (WST-1 PMS) with sarcoma osteogenetic-2 (SaOS-2) human cell line. High cell proliferation on Ti and Ti-64 was observed after 1 and 4 days of incubation. After 7 days, proliferation on Ti-64 was the highest followed by Ti. However, TNZ alloy have less cell proliferation through the entire period of incubation. Fibronectin adsorption is less for Ti and TNZ compared to and Ti-64. In decreasing order albumin adsorption was highest on Ti than TNZ alloy followed by less adsorption on Ti-64. Surface wettability of Ti and Ti-64 showed a high contact angle of between 93-98° compared to 86° TNZ alloy. The surface wettability results correlated well to less fibronectin adsorption on Ti-34Nb-25Zr alloy and highest for Ti-6Al-4V. The Ti-34Nb-25Zr showed less cell proliferation which is believed to be linked with fibronectin adsorption results.