
Properties and Extraction of Crude Pectin Esterase from Potato (Solanum Tuberosum)
Author(s) -
Jing Li,
Wenbin Li,
Shanshan Li,
Guanghui Shen,
Qingying Luo,
Hejun Wu,
Anjun Chen,
Zhiqing Zhang
Publication year - 2019
Publication title -
iop conference series materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/611/1/012060
Subject(s) - esterase , chemistry , isoelectric point , chromatography , extraction (chemistry) , solanum tuberosum , phosphate , enzyme , buffer solution , residue (chemistry) , biochemistry , botany , biology
In order to study the enzymatic properties of potato esterase, the crude enzyme of potato esterase was extracted with phosphate buffer solution, and the extraction conditions were optimized. The optimal condition is: the optimal condition for phosphate buffer solution pH 7.0, water bath time for 30 min, extraction temperature 35°C, and solid-liquid ratio 1:4 (g/mL). The property analysis showed that the potato esterase extracted from phosphate buffer solution was not a single esterase, but a kind of esterase isozyme. At 273nm and 325nm, the potato esterase crude enzyme had two characteristics uv absorption peak, its isoelectric point was near pI 4.4, and its molecular weight was roughly distributed at 16.4, 20.866, 22.73 and 40.286 KDa. In 40 °C ∼ 45 °C, under the condition of pH 7.0, better able to maintain its activity, and seven kinds of metal ions are inhibitory effect to it. This study provides a theoretical basis for further understanding the enzymatic characteristics of potato esterase and its application in pesticide residue detection.
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