Open AccessEffects of different pre-treatment methods on anaerobic mixed microflora for hydrogen production and COD reduction from domestic effluent
Author(s) -
Budhi Primasari,
M Z A Tamin,
Majid Mustafa
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/602/1/012061
Subject(s) - effluent , chemical oxygen demand , chloroform , chemistry , fermentative hydrogen production , pulp and paper industry , activated sludge , biogas , sewage treatment , chromatography , hydrogen production , hydrogen , biohydrogen , waste management , environmental engineering , organic chemistry , environmental science , engineering
Effects of different pretreatment methods on sludge inoculum were evaluated concerning hydrogen (H 2 ) production enhancement and COD (chemical oxygen demand) reduction, using domestic effluent in a batch system. The sludge was taken from a recycled line of the activated sludge reactor. Two types of pretreatment were investigated, heat treatment and chloroform treatment. The experiment was conducted at pH 4-6 and inoculum sizes of microbes were 10%, 20%, and 30% respectively; and experiment without sludge pretreatment was also conducted as control. The result showed that 30% COD reduction was achieved for chloroform pretreatment at pH 3 and 10% inoculum size. For heat treatment, a maximum COD removal of 60% was achieved in the experiment at pH 6 and 10% inoculum size. In chloroform pretreatment, a maximum volume of gas evolved was 3.6 mL, at pH 3 and 20% inoculum size. For heat pretreatment, maximum biogas evolved was 2.1 mL, at pH 3 and 10% inoculum size. The experimental results showed that the pretreatment methods (heat treatment and chloroform treatment) at 35 °C and initial pH 5.5 had a positive influence on H 2 production yield and COD removal efficiency during the fermentative H 2 production as compared to the control experiments (without pretreatment). Heat treatment method was shown to be a simple and useful method for enhancing both H 2 producing and COD removal processes from domestic effluent with highest H 2 yield and COD removal efficiency at 0.314 mmol H 2 /g COD and 86%, respectively.