Open AccessMorphological Characterization and Chemical Bond Identification of Collagen-coated Native Silk Fibroin Fibers Using Chemical Method
Author(s) -
Yangcheng Lü,
Zhixiu Hao
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/585/1/012013
Subject(s) - fibroin , biocompatibility , chemical bond , adhesion , silk , amide , materials science , chemical modification , fiber , surface modification , chemistry , polymer chemistry , chemical engineering , composite material , organic chemistry , engineering
Native silk fibroin (SF) fiber has been widely researched as the materials for tissue engineering scaffolds for its robust mechanical properties. Collagen is the main component of extra cellular matrix (ECM) and has remarkable biocompatibility, so it is often utilized to coat native SF scaffolds for improving the cell adhesion. However, there is lack of feasible methodology for coating collagen on the native SF fibers. In this paper we coated collagen on the native SF fibers by creating amide bonds between the carboxyls from native SF and the amino groups from collagen. Morphological characterization (including 3D surface roughness S q , S pq and S vq ,) and chemical bond identification (including amide bond and carboxyl) of the native SF and collagen-coated SF fibers were performed. The results indicated the chemical method could integrally coat collagen on native SF fibers. The chemical modification on the surface and collagen concentration had no significant influence on the 3D roughness. The coated and uncoated groups had high similarity in position of characteristic peaks of chemical bonds.