Open AccessRenal Function Parameter on Acute Toxicity Test of Kapulaga (Amomum cardamom) Seed Extract in Rat
Author(s) -
Ratih Dewi Yudhani,
Riza Novierta Pesik,
Sarah Azzahro,
Adliah Fithri Anisa,
Rizka Hendriyani
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/578/1/012053
Subject(s) - traditional medicine , herb , antispasmodic , medicine , toxicity , renal function , acute toxicity , creatinine , toxicology , pharmacology , biology , medicinal herbs
Kapulaga ( Amomum cardamom ) is one of herb which is commonly used for spices or medicine, especially the seeds. Previous studies showed that the essential oil of cardamom have antimicrobial, antiinflamatory, analgesic and antispasmodic activities. There was also evidence that A.cardamom has antiartherogenic and antidiabetic activity in rat. Despite the medicinal benefits of A.cardamom , this herb is not yet standardize for herb medicine. For its standardization, A.cardamom has to pass the preclinical and clinical studies to ensure its efficacy and safety profile. The aims of this study was to examine the safety profile of A.cardamom seed extract based on renal parameter function (ureum and creatine levels). Acute toxicity test was conducted based on the OECD 420 Fixed Dose Prosedure guideline that consists of two test steps, preliminary and main tests. For preliminary test, initially with 300 mg/kg BW dose of A.cardamom seed extract, followed by 2000 mg/kg BW. The main test consists of control and treatment group and each group used 5 rats. The rats in both groups were given 2000 mg/kg BW in a single dose of A.cardamom seed extract. The ureum and cretainine levels were assessed at day 14 th using an enzymatic-photometric method. The data were analyzed by independent sample test. The results revealed that the ureum and creatinine levels in control and treatment groups were not statistically different. The mean of ureum levels in the control and tretment groups were 47.24±6.18 and 43.98±6.78 (p=0.45) and the mean of creatinine levels were 0.43±0.04 and 0.36±0.12 (p=0.31) respectively. These results show that a high single dose of A.cardamom seed extract (2000 mg/kgBW) did not toxic in rat based on renal function parameters.