Open AccessFluorescence Imaging of E. coli using CdSe Quantum Dots
Author(s) -
Drishya Mohan,
Abhishek Pathak,
P. Resmi,
Punathil Vasu Suneesh,
T G Satheesh Babu
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/577/1/012107
Subject(s) - quantum dot , fluorescence , optoelectronics , physics , materials science , optics
Rapid and reliable method for the selective detection of pathogenic bacteria is critical in environmental monitoring, disease control, food safety and for the diagnosis of infectious diseases. In this study, we have synthesized hydrophilic fluorescent CdSe Quantum dots (CDSe QDs) and characterized using UV-Vis, IR and fluorescence spectroscopy. Crystallinity of the synthesized Qdots were analysed using XRD. As synthesized CdSe QDs were used for the imaging of buccal epithelial cells and pathogenic bacteria. Selectivity of CdSe QDs to detect E. coli was improved by the functionalization of QDs with glycerol. Easy imaging steps and decreased imaging time with the current procedure shows the potential for biomedical imaging.