Open AccessProcaine as a New Coupling Agent for the Spectrophotometric Determination of Fibrates by Diazotization Coupling Reaction
Author(s) -
Mohammed K. Kahlol,
Muthana S. Mashkoor
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/571/1/012084
Subject(s) - chemistry , hydrochloric acid , molar absorptivity , detection limit , spectrophotometry , amine gas treating , aqueous solution , chromatography , nuclear chemistry , organic chemistry , physics , optics
A sensitive, selective, rapid, simple and accurate of the spectrophotometry determination of fibrates in bulk and in dosage forms. This method depend on diazotization of primary amine group of procaine hydrochloric acid with sodium nitrate and hydrochloric acid followed by coupling with fibrates in aqueous mildly acidic medium to form azo dye stable orange - yellow. Showed obeyed beers law between 0.2-14 ppm, with molar absorptivity 3.01∗10 4 L.mole −1 .cm −1 at 385nm. Sandells sensitivity 0.006mg.cm-1, limit of detection (LOD) 0.018ppm and limit of quantitication (LOQ) 0.058 ppm. This method has been successfully applied to determination of fibrates in bulk and in pharmaceutical preparation, tablet with a good recoveries 99.63-100.25 %