Open AccessHPLC Method for Simultaneous Determination of Six Isoflavones in Soybean Yoghurt
Author(s) -
Zican Huang,
Ming Xia
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/562/1/012132
Subject(s) - daidzin , genistin , daidzein , glycitein , chromatography , isoflavones , chemistry , high performance liquid chromatography , genistein , biochanin a , acetonitrile , biochemistry , biology , endocrinology
Objective: To establish an HPLC method for simultaneous determination of six isoflavones (daidzin, glycitin, genistin, daidzein, glycitein, and genistein) in soybean yoghurt, providing a reference for its development of quality standards and functional evaluation. Methods : The six kinds of isoflavones were measured on a C18 column (250 mm×4.6 mm, 5μm) with a gradient solvent system of acetonitrile - trifluoroacetic acid solution as mobile phase at a flow rate of 1mL·mL-1 and the column temperature was kept at 40°C. The detection wavelength was 255nm. Results : Excellent chromatographic separation and good linearity with peak areas were achieved for 6 kinds of soybean isoflavones composition. The experiment was reproducible. Within the studied concentration ranges, the RSD were less than 2.50% and the average recovery were between 94.10% and 99.70%. Conclusion : This HPLC method is simple, feasible, stable and reliable. More importantly, it can detect the content of six soybean isoflavones simultaneously.