Open AccessPrediction of Ethylene Production Rate of Barangan Banana Using Kinetic Enzymatic Michaelis-Menten
Author(s) -
Ataur Rahman,
E. Darmawaty,
Usman Ahmad
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/557/1/012071
Subject(s) - ethylene , arrhenius equation , rate equation , kinetics , respiration rate , chemistry , thermodynamics , kinetic energy , michaelis–menten kinetics , reaction rate , materials science , mathematics , analytical chemistry (journal) , respiration , activation energy , chromatography , enzyme , biochemistry , enzyme assay , physics , botany , biology , catalysis , quantum mechanics
MM is a model that describes the rate of reaction of product ethylene towards the substrate concentration and MMC is an enzymatic kinetics model to see the effect of the increase of O 2 on the rate of reaction. The objective of the study was to estimate ethylene production rate in bananas by applying the Michaelis-Menten enzymatic kinetics equation. Barangan Banana with harvest age 11 and 10 weeks were measured respiration rate with continuous gas analyzer in temperature 10, 20, and 28 °C with 3 replications along measurement of ethylene production rate using ethylene meter obtained from a closed system method and represented by Michael-Menten equation. The temperature dependence was represented base on Arrhenius law. The model parameters (Km C 2 H 4 dan r C 2 H 4 max ) are calculated using Lineweaver-Burk linier graph method. MM and MMC applications can be used to estimate ethylene production rate of Barangan banana. MM equation resulted that 11-week harvest age obtaining R 2 was higher than 10 weeks 0.95 while the 10-week harvested R 2 0.94. MMC equation, the 11-week harvest age R 2 0.95 was higher compared to 10 weeks R 2 0.94. It could be predicted that MM and MMC equations can predicted the rate of ethylene production of Barangan banana