Open AccessSugar-Rich Hydrolyzates from Coffee Pulp Waste which Produced under Solid State Fermentation by Pestalotiosis sp. VM9 and Aspergillus sp. VTM5, and Its Efficiency as Medium for Single Cell Protein Saccharomyces cerevisiae
Author(s) -
Syafiq Ubaidillah,
Kahar Muzakhar
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/546/6/062033
Subject(s) - chemistry , solid state fermentation , fermentation , food science , sugar , pulp (tooth) , reducing sugar , incubation , saccharomyces cerevisiae , bioprocess , single cell protein , hydrolysis , yeast , yeast extract , pulp and paper industry , biochemistry , biology , medicine , paleontology , pathology , engineering
Polysaccharides rich coffee pulp is one of some abundant agricultural waste in Indonesia. The pulp also contains protein which may lead to be an important source for industrial bioprocessing. Under solid-state fermentation (SSF) by Pestalotiosis sp VM9 using of this pulp, reducing sugar was produced 392.35 μg/ml after 6 days incubation at 30°C. Meanwhile, SSF of the pulp by Aspergillus sp. VTM5, after 5 days of incubation 446.6 µg/ml of reducing was released. The investigation proved that the hydrolyzates medium which were produced under SSF by Pestalotiosis sp VM9 and Aspergillus sp. VTM5 can be used as a source for single cell protein (SCP) Saccharomyces cerevisiae production. The SCP productions using both hydrolyzates were1.89x10 6 2.9x10 6 cell/mL after 54 and 48 hours of incubation at 30°C. Furthermore, during SCP production, S. Cerevisiae consumed sugars as carbon sources in a range of 189.8-225.5 μg/ml (49.5-51.6%) from initial concentration. From the results, it is suggested that the coffee pulp waste can be used as a cheap medium for SCP production. Further investigation to improve SCP production efficiency, optimizing of hydrolysis under SSF and analysis of hydrolyzates component were needed.