Open AccessThe inhibitory effect ofAndrographis paniculataextract on proliferation of breast cancer cell line
Author(s) -
Mentari Maratus Sholihah,
Dono Indarto,
Triyanta Yuli Pramana
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/546/6/062029
Subject(s) - andrographis paniculata , mcf 7 , cell growth , dimethyl sulfoxide , cell culture , chemistry , mtt assay , cell , biochemistry , ethanol , cancer cell , traditional medicine , microbiology and biotechnology , pharmacology , biology , cancer , human breast , medicine , pathology , alternative medicine , genetics , organic chemistry
Aerobic glycolysis is commonly used as the main energy source for cancer cell growth. Adenylate kinase 2 (AK2) plays an important role in maintenance of ATP production in the mitochondria. Neoandrographolide in Andrographis paniculata potentially inhibit AK2 activity in silico . This study aimed to investigate the inhibitory effect of A. paniculata extract on proliferation of breast cancer cell line. This experimental laboratory used MCF-7 cell line and A. paniculata plants, which were extracted using the maceration method with 70% ethanol. Neoandrographolide concentration in A. paniculata extract was determined using HPLC. A total 1x10 4 MCF-7 cells were incubated for 24 hours with or without 0.4% (v/v) dimethyl sulfoxide as solvent (SC) or negative control (NC) group. The same amount of MCF-7 cells was treated with A. paniculata extract with 3 different dosages: 222 ppm (AE1), 111 ppm (AE2) and 55.5 ppm (AE3). The inhibition of cell proliferation used the 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromide (MTT) assay. Data were analyzed using ANOVA and LSD tests with p value <0.05.Ethanol extract of A. paniculata contained 4.43 µM neoandrographolide. The inhibition of MCF-7 cell proliferation was found in AE1 (47.98%) and AE2 (30.50%) groups and was significantly different from the NC group. While the percentage of MCF-7 cell inhibition in the AE1 group differed from NC (p = 0.002) and SC (p = 0.013) groups. Ethanol extract of A. paniculata can inhibit MCF-7 cell proliferation. Further Study is needed to investigate the role of neoandrographolide on inhibition of AK2 activity in MCF-7 cells.