Open AccessEffect of DMSO concentration on the quality of goat fetus fibroblast cell cultured in vitro
Author(s) -
H N Karima,
Trinil Susilawati,
Muhammad Sasmito Djati,
Gatot Ciptadi,
Setiyawati,
Ardyah Ramadhina Irsanti Putri
Publication year - 2019
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/546/6/062011
Subject(s) - cryoprotectant , dimethyl sulfoxide , viability assay , in vitro , andrology , cryopreservation , fibroblast , fetus , cell , chemistry , microbiology and biotechnology , chromatography , biology , biochemistry , embryo , pregnancy , medicine , organic chemistry , genetics
Dimethyl sulfoxide (DMSO) is one of the cryoprotectants that have been used in cell freezing. Cryoprotective agents are necessary to deal with damage in cells associated with slow freezing. Goat foetuses are obtained from local slaughterhouse in Sukun, Malang, East Java. Using various DMSO concentration that were 0% control, 5%, 10%, and 15%. The cell was free with slow freeze methods using Mr. Frosty in -20°C. After 24 days, the samples were analyzed for their viability, size, and abnormality. The result shows the cell size ranged from 3 µm until 5 µm in all samples, categorized as small cell. In viability is significantly influential on 10% DMSO concentration of 92%, while at 0% concentration is 0%, 5% concentration is 56%, and 15% concentration is 89.67%. The abnormality in DMSO 0% all cells are dead and the tissue isn’t developing, in DMSO 5% the abnormality is 3.17%, DMSO 10% is 2.5%, and DMSO 15% 5.67. From the results, we can conclude that the concentration of 10% of DMSO is the best concentration to be used as a freezing method for goat fetus cells.