Open AccessThe validation HPLC method for determination of gambogic acid in gamboge resin
Author(s) -
Ei Mon Khaing,
K Jitrangsri,
J Mahadlek,
C Sukonpan,
Thawatchai Phaechamud
Publication year - 2022
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1234/1/012020
Subject(s) - gambogic acid , chromatography , high performance liquid chromatography , chemistry , acetonitrile , calibration curve , content determination , detection limit , in vitro , biochemistry
Gambogic acid obtained from plant resin of Garcinia species has anticancer properties. This study focuses on the development and validation of the high-performance liquid chromatography (HPLC) method for the quantification of gambogic acid (GA) content in the gamboge raw sample. The chromatographic system was performed on C18 column (150 × 4.6 mm, 5 μm). The system consisted of acetonitrile and 0.1% orthophosphoric acid (85:15 v/v) as mobile phase with a flow rate of 1.5 mL/min. The detection was carried out at a wavelength of 360 nm and the retention time of GA was evident at 9 min. The method was validated according to ICH guidelines. The obtained calibration plot exhibited good linearity in the range of 5 to 120 μg/mL (r 2 > 0.999). The percentage recovery GA spiked in the gamboge sample ranged from 98.87% to 102.92%. The LOD and LOQ were shown to be 2.069 and 6.271 μg/mL, respectively. The validation study demonstrated that this method was simple, specific, and applicable for quantitative analysis of GA in gamboge resin.