Open AccessBiosynthesis of silver nanoparticles by cell-free extract from Trichoderma reesei - study on the influence of growth media
Author(s) -
Orlin Gemishev,
Marinela Panayotova,
Vladko Panayotov
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1117/1/012007
Subject(s) - trichoderma reesei , silver nanoparticle , chemistry , nuclear chemistry , microorganism , biotransformation , biosynthesis , yeast extract , biomass (ecology) , aqueous medium , food science , extraction (chemistry) , growth medium , aqueous solution , nanoparticle , chromatography , biochemistry , fermentation , organic chemistry , nanotechnology , cellulase , bacteria , biology , materials science , enzyme , agronomy , genetics
Trichoderma reesei is a non-pathogenic microorganism able to reduce the toxic for the microorganism silver ions (Ag + ) to non-toxic silver nanoparticles (AgNPs). The effect of components of the nutrient medium on which the biomass from T . reesei was obtained on the biosynthesis of AgNPs from Ag + was monitored. Five media were studied. The main medium (1) contained glucose-2%, NH 4 Cl-0.1%, CO(NH 2 ) 2 -0.3%, (NH 4 ) 2 SO 4 -0.1%, KH 2 PO 4 -0.2%, (NH 4 ) 2 SO 4 -0.14%, MgSO 4 .7H 2 O-0.03%, CaCl 2 .2H 2 O-0.04%. The other were medium 1+0.1% yeast extract (2), medium 1+0.1% corn steep liquor (3), medium 1+0.1% peptone (4), medium 1+0.1% casamino acids (5). After culturing the strain, the resulting biomass was subjected to aqueous extraction, and the cell-free extract - CFE (containing biologically active metabolites) was used for extracellular biotransformation (performed with 10% extracted biomass and 10 mM AgNO 3 ) of Ag + to AgNPs. The AgNPs formation was monitored by UV-Vis. The isolated nanoparticles were characterized by TEM, ICP-AES and FTIR-ATR. The slowest reduction rate of Ag + was obtained with the CFE from biomass grown in medium 1 and the highest - with CFE from biomass grown in medium 3. Nearly mono disperse spherical AgNPs (2-6 nm) were synthesized with the aid of CFE of fungi cultivated in media 1 and 3. The AgNPs obtained with CFE from the other media were 4-11 nm.