Open AccessCalcite mineral characterization and quantification of urease enzyme mediated sand columns
Author(s) -
V Srinivasa Reddy,
K Latha,
Ravulaparthi Sudha Lahari,
V Mallikarjuna Reddy
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1091/1/012027
Subject(s) - calcite , urease , calcium carbonate , chemistry , calcium , urea , carbonate , leaching (pedology) , mineralogy , nuclear chemistry , geology , biochemistry , organic chemistry , soil water , soil science
In this paper urease enzyme facilitated calcium carbonate precipitation for soil strength improvement is characterized using X-ray diffraction and the amount of calcium carbonate precipitated is quantified based on acid leaching, thermal decomposition and CO 2 volume evaluation approaches. These techniques are adopted to evaluate the spreading of calcium carbonate crystals within the sand matrix after treatment with urease enzyme and nutrients. Sand columns of 60mm diameter and height 60 mm are prepared with the help of PVC pipes. Nutrient solution made up of urea, urease enzyme producing Sporosarcina pasteurii bacterium and calcium supplement in the form of calcium chloride is introduced into the sand columns ensuring the uniform distribution. Later treated samples of sand is examined for calcium carbonate precipitation by Sporosarcina pasteurii bacterium by XRD and quantified.