Open AccessThe study of xylanase immobilize enzyme using sol-gel method
Author(s) -
Sigit Hadiantoro,
Yanty Maryanty,
Damar Wulan,
Sastira Putri,
E. M. Putra,
N. S. Achmadin
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1073/1/012010
Subject(s) - xylanase , trichoderma viride , aspergillus niger , bacillus subtilis , chemistry , food science , fermentation , trichoderma , enzyme assay , enzyme , xylan , microorganism , bacteria , microbiology and biotechnology , biochemistry , biology , botany , genetics
Xylanase has many benefits, but many obstacles to produce it. Common microorganisms produce fungi and bacteria such as Aspergillus niger, Trichoderma viride, and Bacillus subtilis. Various methods can do enzyme immobilization; one of them is entrapment in the form of sol-gel. This study discusses the xylanase activity. The enzymes have produced from Aspergillus niger, Trichoderma viride, and Bacillus subtilis. After that, the xylanase enzyme has immobilization using the sol-gel method. This study is doing by lab work and study literature. Lab work is for knowing fiber amount by van Soest method with amount of % hemyselulosa 4,8% 7,33%, 9,1% for media 0 months, two months, and four-month. The study literature doing with compare xylanase amount by Aspergillus niger, Trichoderma viride, and Bacillus subtilis the highest enzyme activity by Trichoderma viride with optimum condition temperature 25°C, pH five and incubation time 36 hours with enzyme activity 26,67 U/mL. The immobilization obtained xylanase activity of 28.88 U / mL. The matrix composition of TEOS, 5% xylan, and 5% calcium alginate during fermentation for 168 hours. This value is higher than the fermentation process without immobilization carried out for 72 hours with an activity value of 22.04 U / mL.