Open AccessIn vitro antioxidant activity of macroalgae Sargassum duplicatum and Palmaria palmata extracts collected from Sepanjang Beach, Gunungkidul, Yogyakarta
Author(s) -
Cici Darsih,
Anastasia Wheni Indrianingsih,
Crescentiana Dewi Poeloengasih,
Dwi Joko Prasetyo,
N Indirayati
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1011/1/012052
Subject(s) - ethyl acetate , dpph , sargassum , antioxidant , chemistry , extraction (chemistry) , polyphenol , food science , botany , chloroform , traditional medicine , algae , biology , chromatography , biochemistry , medicine
Marine macroalgae are considered as important sources for bioactive phytochemicals. In this research, two potential marine macroalgae, i.e. Sargassum duplicatum and Palmaria palmata taken from Sepanjang beach, Gunungkidul, Yogyakarta, Indonesia were explored as sources of phenolic compound, and their antioxidant activities were evaluated. Three different solvents, i.e. chloroform, ethyl acetate, and methanol were applied as extraction medium. For each species, its total phenolic content (TPC) and antioxidant activity were determined using Folin-Ciocalteu reagent and DPPH scavenging assay, respectively. The result showed that methanolic extracts exhibited the highest yield in both species. The highest TPC (726.54 mg GAE/g) was obtained from S. duplicatum extracted using ethyl acetate. The antioxidant activity from S. duplicatum and P. palmata have similar value with IC 50 values of 790.34 µg/mL and 789.29 µg/mL. The scavenging activities of all extracts related to the total polyphenol content, whereas the polarity of solvent affected the resulted yield and TPC of the extracts. However, our results demonstrated that all extracts were inactive against DPPH radicals (IC 50 > 250 μg/mL).