
Anticancer activity of Lampung Robusta Coffee extract fractions
Author(s) -
Husniati Husniati,
Devi Oktiani,
Tati Suhartati,
Valentino Budi Pratama,
Anastasia Fitria Devi,
Nina Artanti
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1011/1/012040
Subject(s) - aroma , column chromatography , chlorogenic acid , chemistry , flavour , fractionation , antioxidant , fraction (chemistry) , food science , methanol , confusion , traditional medicine , chromatography , organic chemistry , medicine , psychology , psychoanalysis
Coffee is a popular beverage that is consumed worldwide. Habitual coffee drinking is associated with cancer risk reduction including pre- and post-menopausal breast cancer. The invert correlation between coffee intake and breast cancer risk is weak, therefore coffee is potential for functional beverage. Coffee should be taken for several times per day to get its benefit. Compounds which give the cancer reduction effect are still debatable, they can be compounds with antioxidant or non-antioxidant properties. Robusta coffee from Lampung has a special meaning for Indonesian coffee lovers due to its strong aroma and flavour with affordable price. In order to observe the anticancer activity of Lampung Robusta coffee, its aqueous methanol extract was passed through a chromatography column containing polyamide with aqueous methanol as the eluent with a gradient of 10%. A total of 54 fractions were obtained and labelled as A to K fractions. Chlorogenic acid was detected in fractions A and B. Meanwhile, it was not detected in other fractions including G fraction. This G fraction, however, was the only one to show cytotoxicity against MCF-7 breast cancer cell line. This result suggests that this fraction contains potential compounds with anticancer activity.