Open AccessEncapsulation of proteins with high/low pI in PLGA-particles for oral delivery
Author(s) -
Riyona Desvy Pratiwi,
Dian Fitria Agustiyanti,
Fitri Agustiani,
Mega Ferdina Warsito,
Apon Zaenal Mustopa
Publication year - 2021
Publication title -
iop conference series. materials science and engineering
Language(s) - English
Resource type - Journals
eISSN - 1757-899X
pISSN - 1757-8981
DOI - 10.1088/1757-899x/1011/1/012026
Subject(s) - plga , lysozyme , polylactic acid , macromolecule , controlled release , chemistry , drug delivery , pi , polymer , biocompatible material , particle size , biophysics , nanotechnology , chemical engineering , materials science , biochemistry , in vitro , biomedical engineering , organic chemistry , medicine , biology , engineering
Polylactic co-glycolic acid (PLGA) is a biocompatible and biodegradable polymer used for controlled drug delivery of small molecules or macromolecules including protein. Proteins highly requires an advanced drug delivery system because of its sensitivity towards many factors such as temperature, hygroscopicity, and biological system. Particularly for oral dosage form, protein must be encapsulated in extremely low pH resistant particle. Protein’s intrinsic characterization determines effectivity of the encapsulation. The recent method of PLGA particle preparation was more suitable for proteins with low pI. PLGA showed higher effectivity to encapsulate protein with low pI such as BSA (pI = ˜ 4.8) than that of high pI for instance lysozyme (pI = ˜ 11). In the study, protein release and physico-chemical characterizations of BSA loaded PLGA-particle were performed.