Open AccessThe Effect of Antiviral Ribavirin, Explant Size, Varieties on Growth and Development in Potato Meristematic
Author(s) -
A.K. Karjadi,
Karjadi,
Neni Gunaeni
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/985/1/012022
Subject(s) - explant culture , plantlet , meristem , shoot , biology , ribavirin , horticulture , botany , virus , virology , in vitro , hepatitis c virus , biochemistry
Potato ( Solanum tuberosum L) is included in the genus Solanaceae, which were propagated by vegetative through tubers. Viral systemic disease is an important seed-borne disease, with tissue culture technique combined with explant size, added antiviral Ribavirin, could be used for eliminated diseases in growth and development. The aim of this study was to determine the effect of antiviral Ribavirin concentrations, explant size and varieties on growth and development of meristematic. The treatment was concentration antiviral Ribavirin (0, 15, 20 mg/l), in MS media, explants size (meristems, shoot tips), varieties (Granola, Medians, Atlantic). The research was conducted from March to September 2019 in the IVEGRI tissue culture laboratory. The results were concluded as follow 1). the percentage of explant shoot tip contamination were higher than the meristem. 2) the average number of shoots, nodes and roots per plantlet from the treatment of varieties, explant size and the added of antiviral Ribavirin has an effect, where the average number of shoot tips were higher than the meristem. 3) The percentage of plantlets abnormal up to 12 WAP was 20 to 50%. 4) The results of the serology DAS ELISA test for both varieties, the added of antiviral Ribavirin and explant meristem has been decreased the number of PLRV infected.