Open AccessIn vitro culture of Zingeria biebersteiniana (Claus) P. Smirn as a method of conservation and expanding its biodiversity
Author(s) -
A V Khromov,
Mikhail Yu. Cherednichenko
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/981/4/042070
Subject(s) - callus , explant culture , organogenesis , botany , murashige and skoog medium , germination , sterilization (economics) , nutrient , sodium hypochlorite , horticulture , plant tissue culture , biology , chemistry , in vitro , ecology , biochemistry , economics , foreign exchange market , foreign exchange , organic chemistry , gene , monetary economics
Zingeria biebersteiniana (Claus) P. Smirn (2n = 4) is an insufficiently explored model plant and its research prospects are wide. At the same time, it is included in the Red List of Russia due to the narrow ecological amplitude of the species. During the work for the first time for this species, the conditions for the induction of callusogenesis and somatic organogenesis were studied. Aseptic plants were obtained by sterilization with 5 % sodium hypochlorite solution; seed stratification increased seed germination rate. Since rooting is often a problem by in vitro cultivation, various concentrations of the main components of MS nutrient medium were studied. It was found that for the induction of root formation the optimal composition of the nutrient medium contains 1/8-1/2 concentrations of mineral salts and vitamins according to the recipe MS. The study of the possibility of induction of callus formation was carried out on seeds and on leaf explants. For induction of callusogenesis on seeds, the greatest effectiveness was shown by MS with the addition of 4 mg/l 2,4-D, for further reproduction of callus - MS with the addition of 0.1-0.5 mg/l 2,4-D; however, it was not possible to achieve callus formation on leaf explants. The highest frequency of somatic organogenesis in callus tissue was achieved on MS with the addition of 1 mg/l BAP and 1/12 mg/l IAA.