Open AccessPreparation of leaf anatomy slide using modification protocols
Author(s) -
A N Mardiyyaningsih,
E Daningsih
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/976/1/012061
Subject(s) - protocol (science) , computer science , safranin , anatomy , staining , significant difference , biomedical engineering , biology , mathematics , pathology , medicine , statistics , alternative medicine
Preparing leaf anatomy slide is a routine procedure in physiology and anatomy plant research. A standard microtechnique method frequently adopted for doing such procedure is Johansens’. However, for early user such as university students, executing this method may be challenging in term of using many chemicals in different stages which make it more pricey and need longer duration. This research attempts to analyse the possibility for using simpler protocol suggested by Gunarso to replace Johansens method. It is conducted under a qualitative experiment design involving leaves from monocotyl and dicotyl classes as samples. Samples are analysed with safranin-fast green staining protocols. Result shows that there is no difference between Johanssens’ and Gunarso modified protocol (GMP) with respect to vivid observable of the cell membrane, the tissue structure, and the colour intensity. Hence, this new modification protocol is potential to replace the Johansens methods in leaf anatomy slide