Open AccessCoating formulas protect shelled nutmeg seeds from Aspergillus colonization
Author(s) -
Supriadi Supriadi,
St. A. Rahayuningsih,
Siti Hardiyanti,
B. Sofianna Sembiring,
Ediningsih
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/974/1/012112
Subject(s) - aspergillus flavus , nutmeg , aflatoxin , potassium sorbate , food science , methylparaben , chemistry , propylparaben , inoculation , horticulture , toxicology , biology , preservative , sugar
Coating of nutmeg seeds may prevent the colonization of Aspergillus contamination. The study aimed to evaluate the effect of coating formulas to A. flavus on shelled nutmeg. Shelled nutmeg seeds were coated with aqueous (TT) and gel (GM+) formulas containing propylparaben (0.1%), potassium sorbate (0.8%), and clove oil (1.25%), and the aqueous GM- (0.65% clove oil and 0.4% potassium sorbate). The coated seeds were then sun-dried and sprayed with A. flavus conidia. The untreated control was only inoculated with A. flavus . The colonization of A. flavus on the seeds was visually observed. The aflatoxins, propylparaben, and potassium sorbate in the treated seeds were analyzed with HPLC. The results showed that seeds treated with GM+, GM-, and TT formulas were visually free from A. flavus . The total aflatoxins were not detected in the seeds treated with the GM+, but in the coated with GM- and TT was 0.95 µg/kg and 12.6 µg/kg, respectively. In the uncoated seeds, the total aflatoxins were 695.9 µg/kg. Propylparaben and potassium sorbate residues in the coated seeds were 66-11 mg/kg and 213-415 mg/kg, respectively. The coating formula effectively minimized A. flavus colonization and aflatoxin. Therefore, the coating formula could be used for reducing Aspergillus contamination.