Open AccessMolecular characterization of Spodoptera litura Nucleopolyhedrovirus (SlNPV) Labuhan Batu isolate
Author(s) -
D P Sinambela,
Darma Bakti,
Maryani Cyccu Tobing,
Y M Kusumah
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/974/1/012011
Subject(s) - spodoptera litura , biology , amplicon , veterinary medicine , pest analysis , polymerase chain reaction , horticulture , microbiology and biotechnology , botany , lepidoptera genitalia , medicine , gene , genetics
Spodoptera litura F. is a new pest that potentially becomes a serious pest threatening palm oil industry. The control can be carried out using biological control agents S.litura Nucleopolyhedrovirus ( Sl NPV). Molecular approach using polymerase chain reaction (PCR) has been used to characterize the Sl NPV isolate. The aim of this study was to determine the molecular characteristics of Sl NPV Labuhan Batu isolate based on DNA sequence analysis. The NPV isolate was collected from oil palm plantation Negeri Lama Estate Group, PT. Hari sawit Jaya, Asian Agri, Labuhan Batu. The Sl NPV DNA extraction was conducted using the DNA gSYNC kit. The amplification of Sl NPV DNA was done by specific primers with oligonucleotide sequences forward 5′-ACGTTACGATCTAGATCC-3′ and reverse 5′-AACGTTAGACAGACATAT-3′ with ±700bp of amplicon. Homology and phylogeny characteristics were used to determine the relationship of Sl NPV isolate from Labuhan Batu to NPV from other countries were reported in the GeneBank of National Center for Biotechnology Information (NCBI). The results showed that Sl NPV isolate from Labuhan Batu had the closest related to Sl NPV from Australia, Sli NPV from Canada and Sl NPV from China, with the similarity percentage 100%, 96% and 90% respectively. They belong to the same group as the NPVs that infected the Spodoptera genus.