The effect of ultrasonic extraction methods on extract quality from Strobilanthes crispus L.

Strobilanthes crispus L. leaves are known to have many health benefits that have been proven both traditionally and in scientific research. To achieve more benefits from the leaves of Strobilanthes crispus L., the ultrasonic extraction (53 kHz) method was carried out using various ways. The variations of the ultrasonic method being carried out are UAE (Ultrasound-Assisted Extraction), UAEE (Ultrasound-Assisted Enzymatic Extraction), UAE-ATPE (Ultrasound-Assisted Enzymatic Aqueous Two-Phase Extraction) sequence and simultaneously. Extraction was carried out at 27 °C for 1hour, enzymatic hydrolysis reaction for 2 hours with a cellulose concentration of 7% w/w dry leaves, and the ATP (Aqueous Two-Phase) system of ethanol-ammonium sulfate of 33-14% w/w. The extract parameters measured were the yield, TPC (Total Phenolic Content), and TFC (Total Flavonoid Content) values. The highest yield was obtained by the Hydrolysis/UAE-ATPE method simultaneously, namely 80.94% w/w dry biomass. The highest TPC and TFC values were obtained from the extraction using the Hydrolysis/UAE-ATPS method simultaneously, i.e. 5.517 and 3.332 mg/g dry biomass respectively. Thus, it can be concluded that the simultaneous use of hydrolysis enzymatic with the cellulase enzyme and the ATP system in the ultrasonic extraction method can increase the quantity and quality of Strobilanthes crispus L. leaves extract.