Open AccessGrapes recovery from viruses during reproduction by biotechnological method
Author(s) -
Abdulmalik Batukaev,
Д.О. Палаева,
М.С. Батукаев
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/954/1/012010
Subject(s) - biology , phytoplasma , virus , pathogen , virology , mosaic virus , plant virus , botany , polymerase chain reaction , horticulture , microbiology and biotechnology , gene , genetics , restriction fragment length polymorphism
Grape regenerant plants obtained from apical meristems were assessed for the presence of the viruses using PCR and enzyme-linked immunosorbent assay (ELISA). The vast majority of the samples studied for the presence of the most common viruses ( Grapevine Leafroll-Associated Virus – 1 (GLRaV-1); Grapevine yellow mosaic virus; Grapevine vein banding; virus; Grapevine Leafroll Virus; Grapevine stemm pitting ) by ELISA showed negative results. Testing of regenerant plants of the Augustine variety clones showed that only one plant out of 80 plants showed a positive reaction to the presence of this virus. Analysis of 80 plants of the Moldova variety clones revealed a positive reaction to the yellow mosaic virus ( Grapevine yellow mosaic virus ) in 1 plant as well as in the original donor plant (Moldova variety). The rest of the regenerated plants (79 pcs.) were absolutely healthy. PCR analysis showed that the spectrum of fragments of the original genotypes of Moldova varieties (lanes 1–4) and Augustin (lanes 5–8) contained DNA fragments of 450 bps, corresponding to Candidatus Phytoplasma vitis Flavescence doree . In Bart variety (lanes 9–11), no pathogen was detected. Analysis of the spectrum of fragments of grape regenerant plants obtained from apical meristems (lanes 1–4) and Augustine (lanes 5–8) showed the absence of the pathogen Candidatus Phytoplasma vitis Flavescence doree.