Open AccessAssessment cytotoxic assay of Rhizophora plants mangrove using brine shrimp (Artemia salina L) model
Author(s) -
I Indriaty,
Binawati Ginting,
Kartini Hasballah,
DJUFRI DJUFRI
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/951/1/012070
Subject(s) - rhizophoraceae , brine shrimp , rhizophora mucronata , mangrove , artemia salina , biology , rhizophora , botany , bark (sound) , bioassay , chemistry , toxicity , ecology , organic chemistry
Rhizophoraceae is the main family of mangroves as a source of bioactive compounds originating from the coast. Ethnophamacologically Rhizophoraceae has been used in various traditional medicine. Natural sources as anticancer from the Rhizophoraceae family are interesting to know. This study aimed to determine the cytotoxic bioactivity of methanolic extracts of roots, bark, leaves, and fruit/hypocotyl from five species of Rhizophoraceae (Bruguieria cylindrica, B. gymnorrhiza, Ceriops decandra, Rhizophora apiculata, and R. mucronata) from the Langsa mangrove forest, Aceh. The method used in this study was the Brine Shrimp Lethality Test (BSLT) bioassay using Artemia salina Leach at extract concentrations of 1, 10, 100, 500, and 1000 μg/ml. Samples were extracted using the maceration method and methanol as the solvent. The cytotoxic activity of 20 Rhizophoraceae methanol extracts showed that 12 extracts were toxic with an LC50 range of 31.5 - 934.9 μg/ml (based on LC 50 ≤ 1000 μg/ml). The two extracts of which the closest to highly toxic (based on LC 50 ≤ 30 μg/ml) were C. decandra bark showed LC 50 of 31.5 μg/ml, and R. mucronata bark showed LC 50 31.8 μg/ml. This shows that Rhizophoraceae extract has potential as a natural anticancer agent. In the five rhizophoraceae species, C. decandra was the most active compared to other species. In the four plant parts, the bark was the most toxic.