Open AccessAntagonistic screening of Trichoderma spp. isolated from patchouli rhizosphere
Author(s) -
Muzakir,
Hifnalisa Hifnalisa,
Jauharlina Jauharlina,
Rina Sriwati
Publication year - 2022
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/951/1/012021
Subject(s) - patchouli , pogostemon , rhizosphere , fusarium , trichoderma , biology , horticulture , agar plate , agar , potato dextrose agar , botany , veterinary medicine , traditional medicine , bacteria , medicine , genetics , essential oil
The objective of this research was to determine the antagonistic activity of Trichoderma spp. isolated from patchouli rhizosphere (Pogostemon cablin Benth.). Another objective was to perform antagonistic screening of these fungi to inhibit the growth of the wilted pathogen Fusarium spp. In vitro research was conducted in the Laboratory of Plant Pathology, Universitas Syiah Kuala, from January to June 2020. The study used a completely randomised design with five treatments and three replications. The antagonistic screening was carried out by using the dual culture method of Trichoderma spp. against Fusarium spp. with the medium of Potato Dextrose Agar (PDA). The result showed that five isolates of Trichoderma have different antagonistic percentages in inhibiting the Fusarium. The highest antagonistic activity was found from isolate 2 and the lowest value was shown by isolate 3.