Open AccessDEVELOPMENT OF THE BLACK SEA HYDROBIONT RESOURCE STUDY METHOD AS A NEW ECONOMICALLY FEASIBLE DIRECTION OF PHARMACOLOGY AIMING TOWARD SAVE HUMAN HEALTH AND ECOLOGY
Author(s) -
O. P. Chzhu,
D. E. Araviashvili,
A L Tumanova
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/941/1/012023
Subject(s) - chlorella vulgaris , biochemical engineering , biological activity , extraction (chemistry) , raw material , black sea , resource (disambiguation) , bioassay , environmental science , chemistry , biology , computer science , ecology , algae , biochemistry , chromatography , in vitro , computer network , oceanography , engineering , geology
The selection of the Black Sea aquatic area as a hydrobiont biologically active compound source allows resolving several environmental problems including development of an integrated hydrobiological resource management system for the Black Sea as a new sector of modern pharmacology. Aquatic organisms produce unique secondary metabolites. This paper presents the results of studies on the development of the biologically active substance extraction technology from non-commercial aquatic organisms as well as a preliminary assessment of the biochemical activity of the substrates obtained. Biologically active substances were extracted using the of two-phase extraction method in conjunction with ultrasound. For the substrates, the antioxidant activity was determined utilising the method that allow conducting screening of pharmaceutical raw materials and biologically active substances with high antioxidant activity. The protective activity evaluation was carried out during the study of the enzymatic alcoholic fermentation kinetics within a self-contained system. The evaluation of lymphocytes proliferative activities influenced by the obtained substrates was carried out using the cultivation method. The acquired data allows recommending the two-phase extraction method combined with ultrasonic voiceover as the effective one when processing analogical marine raw materials. The isolated substrates are characterised by a pronounced biochemical activity in relation to the living systems cells, which suggests a significant synergistic effect with derivatives of the microalgae Chlorella Vulgaris.