Open AccessReview of digital PCR potential for surveillance of emerging disease from wastewater
Author(s) -
A Dewantoro,
W C Anggundari,
B Prasetya,
- Yopi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/926/1/012065
Subject(s) - digital polymerase chain reaction , wastewater , sewage , pandemic , covid-19 , infectious disease (medical specialty) , standardization , sewage treatment , biology , microbiology and biotechnology , virology , environmental science , disease , computer science , environmental engineering , polymerase chain reaction , medicine , pathology , biochemistry , gene , operating system
Emerging infectious diseases (EID) such as COVID-19 had been widely caused massive impact for all countries in the world. The spreading of pathogens became uncontrolled and unpredictable to overcome this pandemic disease. Some non-waterborne EID also was discovered in wastewater in many countries of the world. Studies showed that digital PCR could become a powerful tool for environmental surveillance. It enables the performance of absolute quantification for nucleic acid with a high inhibitory sample, like wastewater, and potentially possibly detected a tiny quantity of pathogen residue and tracked the infectious diseases that originated from human excretions into sewage. Hopefully, with the development of this method and support of measurement and standardization, it is possible to become an effective method to overcome the digital PCR (dPCR) method challenge for surveillance of disease transmission from wastewater.