Open AccessIn Vitro Detection of Some Active Compounds in Stressed Callus of Mungbean (Vigna Radiata L.)
Author(s) -
Ekhlas. A.J. ElKaaby,
Hassan A. A. Al-Saady,
Wifaq A. M. Al-Kaisy
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/923/1/012048
Subject(s) - callus , ferulic acid , gallic acid , chemistry , brassinolide , radiata , chlorogenic acid , vigna , food science , tannic acid , botany , horticulture , traditional medicine , biochemistry , biology , antioxidant , organic chemistry , medicine , plant growth
An experiment was conducted at the Ministry of Sciences and Technology/Directorate of Agricultural Research, Genetic Engineering Department. In Vitro callus cultured on (MS) medium supplemented with different levels of NaCl, brassinolides and vitamin C. H.P.L.C technique was used to detect the phenolic compounds in different treatments of mungbean stressed callus. The results showed various responses for accumulation of phenolic compounds in different treatments of mungbean stressed callus for example, 15dS.m −1 NaCl+40mg.L −1 vitamin C+0.04mg.L −1 brassinolide) accumulated highest concentration of Hydroxybenzonic acid, Tannic acid, Gallic acid, Quercetin and Syrinigic acid (280.44, 135.16, 286.49, 778.93 and 778.93 μg.ml −1 ) respectively, while highest accumulation of Chlorogenic acid, Gentisic acid, Sikimick acid and Coumaric acid were found in (6dS.m −1 NaCl+40mg.L −1 vitamin C+0.04mg.L −1 brassinolide) treatment reached (230.27, 208.21, 846.52 and 522.62 μ.mg −1 )respectively. Furthermore, 6dS.m −1 NaCl+0.04mg.L −1 brassinolide and 15dS.m −1 NaCl+0.04mg.L −1 brassinolide achieved highest accumulation (933.83 and 407.48 μg.ml −1 ) for Ferulic acid and Protocatechuric acid respectively.