Open AccessGenetic diversity of Pericopsis mooniana from South Kalimantan based on Random Amplified Polymorphism DNA (RAPD) Markers
Author(s) -
Vivi Yuskianti,
Purnamila Sulistyawati
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/914/1/012029
Subject(s) - rapd , genetic diversity , biology , orchard , seed orchard , inbreeding depression , forestry , inbreeding , veterinary medicine , horticulture , ecology , geography , population , demography , sociology , medicine
The genetic diversity and relationship of Pericopsis mooniana collected from Pulau Laut, South Kalimantan, was analyzed using RAPD markers. Currently, the natural distribution of P. mooniana in Pulau Laut no longer exists. Instead, PT Inhutani II, as the concession holder in the area, had collected the remaining P.mooniana and planted it in the seed orchard, arboretum, or around PT Inhutani’s mess/office. Forty-two samples from four locations: mess/residential house (3), seed orchard (33), arboretum (3), and PT Inhutani II Unit Stagen (3) were analyzed using seven RAPD primers. Our study showed that the total genetic diversity of all populations was low ( He = 0.191 ± 0.013). P. mooniana collected from seed orchards had the highest diversity ( He = 0.289 ± 0.021), while the lowest was from mess/residential houses ( He = 0.134 ± 0.025). The genetic relationship data indicated the possibility that P. mooniana from mess/residential house, arboretum, and seed orchard may come from the same origin. These results can be used to support the development of ex-situ conservation plots to avoid inbreeding depression. At the same time, the genetic diversity will be helpful in its conservation and further utilization, such as establishing the plantations to reduce the pressure of the species in nature and provide valuable timber production in Indonesia.