Open AccessCytotoxicity of Begonia medicinalis aqueous extract in three cancer cell line
Author(s) -
Bunga Prihardina,
Sri Fatmawati
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/913/1/012084
Subject(s) - flavonoid , chemistry , extraction (chemistry) , hela , cytotoxicity , mtt assay , traditional medicine , quercetin , botany , chromatography , biochemistry , biology , in vitro , medicine , antioxidant
Begonia medicinalis or known as benalu batu in Indonesia is a herbal plant that is locally used for traditional medicines. The secondary metabolites such as flavonoids, alkaloids, steroids, and terpenoids have been reported to be found in these plant extracts. The content of flavonoids can lead to anti-cancer abilities while heat-sensitive flavonoid compounds can be extracted by the Ultrasound-assisted Extraction (UAE) method. In this study, the anticancer potential of B. medicinalis extracts from the leaves (leaves extract/LE) and stem (stem extract/SE) in three cell lines (Hela, MDA-MB, HT-29) have been performed. Extraction of the leaves and stems was carried out using water as a solvent and the ultrasound-assisted extraction (UAE) method followed by measuring the total flavonoid content (TFC) of each extract. The anticancer potential was obtained from cytotoxic measurements by the MTT method on 3 types of cancer cells incubated with the extract for 24 hours. The value of total flavonoid content (TFC) in the LE was higher than that of SE extracts. Both extracts have the potential as a remedy for the treatment of cancer. Keywords: Begonia medicinalis , Ultrasound-assisted Extraction (UAE), anticancer