Open AccessDetermine The Genetic Variation of Phenotypic Groups of Quail (Different With Feathers Color ) Using The Chain Polymerase Reaction Technique (PCR)
Author(s) -
Majid A.S. Alneemy,
Esraa Mohammed Tawfeq,
Haitham Mohammed Sabeeh Hadi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/910/1/012110
Subject(s) - quail , feather , genetic similarity , biology , plumage , rapd , similarity (geometry) , genetic variation , agarose gel electrophoresis , genotype , genetic distance , dna extraction , veterinary medicine , genetics , polymerase chain reaction , zoology , dna , genetic diversity , ecology , gene , sociology , computer science , image (mathematics) , medicine , population , demography , artificial intelligence
The main objective of this research was to specify the hereditary variation associated with the plumage color in three local genotypes of Japanese quail which bred in two geographical locations. The birds distributed on six treatments with five birds for each depending on the feather's color and geographical locations. DNA extraction was executed from the blood samples of each treatment and amplified by thermo cycler apparatus and the electrophoresis was done using 1.5% agarose gel for DNA bands exhibiting. Genotype influence has been shown that the black color quail B1 of the agricultural research station in the city of Mosul showed maximum genetic similarity with the Black quail B2 of Tikrit University with a value of 0.9549, the highest genetic similarity between different colors found between B1 and W2 that amounted to 0.9391 based on the similarity index (band sharing). While, the least genetic similarity observed between B2 and W1, which went down to 0.8468. Genetic difference values among studied quail groups showed that the groups B2 and W1 in the higher genetic variation, whereas the least genetic difference found between B1 and B2 groups. The average of dissimilarities for each group with all others varied between the values 0.1203 - 0.0851. The present work prove that the effectiveness of RAPD markers in knowing the similarity and specify the inherited relationship within the quail varieties.