Open AccessAnalysis of metal accumulation and Phytochelatin Synthase (PCS) gene expression in Saccharum spontaneum L. as regards its function for remediation of lead (Pb) contamination
Author(s) -
Dwi Setyo Rini,
Nuril Hidayati
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/905/1/012117
Subject(s) - environmental remediation , phytoremediation , shoot , perennial plant , pollutant , chemistry , contamination , phytochelatin , pollution , horticulture , cadmium , environmental chemistry , botany , heavy metals , biology , biochemistry , glutathione , enzyme , organic chemistry , ecology
Pollution caused by heavy metals, has become a serious problem. Adverse effects arising from the increased use of heavy metals in a variety of human activities lead to any environmental degradation. Lead (Pb) is one of most common contaminants in the environment and highly toxic. Pb is less mobile, so its compound tends to accumulate in soil and sediments. Definitely, efforts are needed to remove this contaminant in the environment. Saccharum spontaneum L. is a perennial grass which has potential to be used as an accumulator plant to clean up pollutants. The ability of this plant as metal accumulator was tested in this study. S.pontaneum plants were treated using Pb in the concentrations of 0 ppm (control), 100 ppm, 200 ppm, and 300 ppm for 8 weeks. The results showed that there was an increase in the percentage of relative accumulation of Pb in the treated plants. This also indicated that plant roots accumulated more Pb than shoots. Meanwhile, expression of Phytochelatin synthase (PCS) gene increased 1.3-to-3.5-fold inductions in roots by increasing concentration of Pb treatments for 24 h. PCS gene expression showed the higher induction in the roots than in the shoots of S.spontaneum plant under Pb treatments.