Open AccessMutation induction in the pineapple (Ananas comosus L. Merr) using colchicine
Author(s) -
Rosmaina,
Rita Elfianis,
F Mursanto,
Akopian Janna,
T Erawati,
Yani Liu,
N N W M Solin,
Zulfahmi Zulfahmi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/905/1/012082
Subject(s) - ananas , colchicine , horticulture , biology , genotype , botany , genetics , gene
Pineapple is a tropical fruit that has high economic value. Mutation is a method to increase plant diversity which plays an essential role in plant improvement. This study aimed to induce mutations in pineapple using colchicine. This study was arranged in a factorial completely randomized design. The first factor was pineapple genotypes (i.e., Gemilang, Bangka, Queen, and Suska Kualu) and the second factor was colchicine concentration (i.e., 0.03%, 0.04%, 0.05%, and control). There were 16 treatments with five repetitions so the total was 80 experimental units. Observations were made for three months after treatment in the vegetative phase. Observation parameters included leaf shape, leaf color, plant height, number of leaves, leaf width, and length of stomata. This study indicated that the interaction between genotype and colchicine significantly differed in plant height and the number of leaves. Colchicine significantly increased the length of stomata, and genotype significantly affected all observed parameters. This study concluded that 0.05% colchicine significantly increased plant height (26.67%) and the number of leaves (48.98%) in the Gemilang genotype but decreased plant height and leaf number in other genotypes. This study suggests the need for observation of the flowering phase and fruit quality due to colchicine treatment.