Open AccessStudying the Effectiveness of Anise Extract and Alignite Nanoparticles on the Balance of the Microbial Gut Flora and Some Immunological Parameters in the Laboratory Guinea Pigs
Author(s) -
Fatimah S. Dawood,
Karkaz M. Thalij
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/904/1/012020
Subject(s) - antibiotics , antimicrobial , metronidazole , biology , diarrhea , microbiology and biotechnology , cefotaxime , flora (microbiology) , guinea pig , feces , gut flora , bacteria , immunology , medicine , endocrinology , genetics
The study aims to determine the effect of oral dosage of anise fruit alcoholic extract and Alignite Nanoparticles (AlgNPs) and their interaction with the antibiotics Cefotaxime and Metronidazole concerning the parameters related to the balance of intestine normal flora with some immunological parameters in laboratory guinea pigs induced with diarrhea by E.coli . The results show that the Enterobacteriaceae bacterial colonies counts belonging less than those accounts of lactic acid bacteria in the intestines of laboratory guinea pigs. It is also found that the synergistic action of the nanocomposites with different antibiotics like what: have a better antimicrobial effect than the effect of each individually. There is also a significant increase (p<0.05) in the IgM and IgA values, which are at 1789 and 362 ng/L, respectively compared with their levels in the control group animals which are at 1230 and 184.5 (ng/L), respectively. The value of IgG does not change significantly in the oral dosage of the extract or the AlgNPs. After the recovery status of laboratory animals from infection, the level of IgG increased significantly, which gives evidence of bacterial infection in the infected animals.