Open AccessSpirulina water extract and Lactobacillus bulgaricus FNCC– 0041, Streptococcus thermophilus FNCC–0040 secretion as immunostimulants in gnotobiotic Artemia challenge tests against pathogenic Vibrio parahaemolyticus, V. vulnificus, and V. harveyi
Author(s) -
Ervia Yudiati,
Sri Sedjati,
Nuril Azhar,
WA Oktarima,
Zaenal Arifin
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/890/1/012018
Subject(s) - vibrio vulnificus , vibrio harveyi , microbiology and biotechnology , vibrio parahaemolyticus , streptococcus thermophilus , lactobacillus , biology , vibrio , immunostimulant , food science , bacteria , fermentation , immune system , immunology , genetics
Spirulina water extract (SWE) has a good potency as an immunostimulant. Lactobacillus bulgaricus & Streptococcus thermophilus are lactic acid bacteria (LAB) that produce exopolysaccharide exudate. Vibriosis is a common infectious disease for aquatic cultivans caused by Vibrio spp. This study determines the ability of SWE in combination with L. bulgaricus & S. thermophilus as immunostimulant (Artemia challenge test) assay against Vibrio parahaemolyticus, V. vulnificus, and V. harveyi . Factorial Design with two factors namely SWE doses (0, 300, 600, and 900 ppb) and Vibrio spp. treatment (non -Vibrio spp., Vibrio harveyi (Vh), Vibrio parahaemolyticus (Vp), Vibrio vulnificus (Vv), Vh-Vp, Vv-Vp, Vp-Vh, and Vp-Vv-Vh) were applicated. SWE was diluted and LAB at a concentration of 10 8 cell/mL were fermented in three days at 30°C. Ten newly hatched Artemia nauplii were enriched with fermented and non-fermented SWE for one hour, then challenged with 10 8 cell/mL Vibrio spp. The survival of Artemia was recorded every 6 hours. Results showed that the survival rate of Artemia enriched with 300 ppb concentration of SWE and LAB was significant than control ( p <0.05). It is concluded that there is a positive effect on the bioencapsulation of the minimum concentration of SWE and LAB secretion to accelerate Artemia’s immune response.