Open AccessAllele and genotype variation of follicle stimulating hormone receptor gene of Indonesian Friesian Holstein in Boyolali District, Central Java
Author(s) -
Galih Pambuko,
Rebecca Vanessa,
Purwadi Purwadi,
N Widyas,
Sigit Prastowo
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/888/1/012011
Subject(s) - genotype , biology , allele , restriction fragment length polymorphism , genetics , genotype frequency , polymerase chain reaction , allele frequency , gene
Follicle Stimulating Hormone Receptor (FSHR) is a gene that has function to regulate reproductive performance by controlling oogenesis in females and also spermatogenesis in male. Since dairy cattle operation lies on the reproductive trait efficiency, therefore it’s became a logical reason to use FSHR genes as part of selection criteria. This study aimed to determine allele and genotype variation of FSHR gene in local Indonesian Friesian Holstein (IFH) dairy cattle in Boyolali District, Central Java. In total 20 IFH cattle we sampled for DNA source. Allele and genotype variation was determined by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using AluI restriction enzyme. The methods started with DNA extraction, PCR, and DNA digestion, followed by allele and genotype frequencies calculation. Two alleles were observed in this study, C and G with respective frequencies 0.75 and 0.25. According to that, we found three genotype combinations that are CC, CG, and GG, the frequencies were 0.56, 0.38, and 0.06, respectively. To use that result as a part of selection criteria in dairy cattle, especially in females, further association study with reproductive trait needs to be done.