Open AccessSNPs detection in 5’-UTR region of the MC4R gene in Garut sheep
Author(s) -
Aprilianna Putri Zahara Nafsina Luvita Sari,
Ichda Rufaida Athifa,
. Panjono,
Riyan Hidayat,
A Barli,
Dyah Maharani
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/888/1/012001
Subject(s) - genotype , single nucleotide polymorphism , biology , allele , genetics , allele frequency , genotype frequency , melanocortin 4 receptor , snp , polymorphism (computer science) , population , gene , microbiology and biotechnology , receptor , medicine , melanocortin , environmental health
The melanocortin-4 receptor (MC4R) gene has been linked to controlling feeding behavior and body weight. The objective of this study was to detect the polymorphism within the 5’-UTR region of the MC4R gene in Garut sheep. A total of 36 blood samples were isolated and amplified using primers (forward: 5’-TTCGTTTGGGGCAAGTCAAG-3’ and reverse: 5’-GGAAACGCTCACCAACATGT-3’). Two SNPs, g.396C/T and g.399C/G, were discovered in the 5‘UTR region based on sequence alignment (position number based on Genbank acc no. NC_040274). For both SNPs, only two genotypes were found in the samples. Both SNPs had identical allele and genotype frequencies. The C allele (86%, n=26) was higher than the T (g.396C/T) and G allele (g.399C/G) (14%, n=10). The homozygous CC genotype has a higher frequency (72%) in both SNP g.396C/T and g.399C/G, followed by CT and CG genotype (28%), respectively. The Hardy-Weinberg equilibrium analysis resulted in the sample population did not deviate (χ2<5.59). Further analysis could be suggested to provide an overview of this polymorphism effect in Garut sheep’s growth traits.