Open AccessEthanol extract analysis of steam pineapple (Ananas comosus. L) and its application as antibacterial agent: In vitro and silico studies
Author(s) -
Nur Istiqomah,
Ardi Ramadhani,
Riska Surya Ningrum,
Endang Purwati
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/886/1/012019
Subject(s) - chemistry , betulinic acid , ferulic acid , antibacterial activity , cinnamic acid , vanillic acid , food science , biochemistry , biology , bacteria , genetics
Pineapple contains active compounds, such as phenolics, steroids, flavonoids, terpenoids, saponins, quinines, coumarins, alkaloids, and tannins. These compounds can be used as antioxidants, antibacterial, and antifungals. The purpose of this study was to determine the activity of compounds in pineapple steam in inhibiting the growth of Staphylococcus aureus, Shigella dysentry, Salmonella typhi in vitro and silico. The extraction method was carried out by maceration using 70% ethanol. Analysis of the compound in pineapple extract using LC-MS. In vitro antimicrobial activity test using the disc diffusion method. Toxtree software was used to test the toxicity of compounds. Physicochemical test of compounds using Lipinski Test. Docking molecular using PyRx software. Visualization of docking results using Discovery Studio 2019 Software. The results showed that the yield obtained from the extraction process of pineapple weevils was 79.3%. There were 58 compounds obtained from the LC-MS analysis. Compounds that have antibacterial activity are acetic acid, cinnamic acid, oxalic acid, malic acid, thiamine, niacin, riboflavin, p-coumaric acid, caffeic acid, ferulic acid, sinapic acid, quercetin, ananasic acid, subaphyllin, beta carotene, lutein, cyamin, cyaniding 3,5,3'-triglucoside, beta-sitosterol, campesterol, and hydroxysitosterol. Toxicity test results with toxtree software found that all compounds belong to Class I. Class, I compound that have low toxicity properties.