Open AccessMycorrhizal fungi Glomus spp. propagation in zeolite enriched with mycorrhiza helper bacteria for controlling nematode in coffee
Author(s) -
Iis Nur Asyiah,
Reginawanti Hindersah,
Rita Harni,
Betty Natalie Fitriatin,
Widya Mayang Anggraeni
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/883/1/012021
Subject(s) - microbial inoculant , biology , inoculation , glomus , spore , horticulture , population , nematode , bacillus subtilis , botany , bacteria , ecology , demography , genetics , sociology
Arbuscular mycorrhizal fungi (AMF) play a role in suppressing the nematode Pratylenchus coffeae. Mycorrhizal helper bacteria (MHB) can increase the effectiveness of AMF to control the diseases. The experimental purpose was to increase the spore population of AMF Glomus spp. in zeolite-based formulation inoculated with liquid consortia of Pseudomonas diminuta and Bacillus subtilis as MHB. The experimental design was a completely random design with six treatments consisted of 10 6 , 10 7 , 10 8 , and 10 9 CFU/mL MHB liquid inoculants. The control treatments were water and 2% molasses. All treatments were replicated four times. A total of 300 mL/pot Liquid inoculant of MHB have been inoculated a three day before transplanting the maize seedling to the Zeolite inoculated with Glomus spp. in the pot. One month after MHB inoculation, Glomus formulation in Zeolite with different levels of MHB increased the degree of infection. Three months after MHB inoculation, spore content in Zeolite increased. The density of P. diminuta and B. subtilis in zeolite-based mycorrhizal inoculant increased at the end of the experiment. Liquid inoculant MHB contained 10 8 CFU/mL enhanced spora number fourth times compared to the control. This experiment suggests that P. diminuta and B. subtilis were effective to increase the spore density of AMF inoculant.