Open AccessModification of Cu2+ in polyphenol oxidase extract from purple eggplant for phenol degradation in coal wastewater treatment
Author(s) -
Anceu Murniati,
B. Buchari,
Suryo Gandasasmita,
Zeily Nurachman,
VA Kusumaningtiyas,
Senadi Budiman,
S Herlinawati,
Ruaa M. Ibrahim,
Djoko Hadi Prajitno
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/882/1/012071
Subject(s) - phenol , polyphenol oxidase , chemistry , biodegradation , phenols , solanum , wastewater , catechol , polyphenol , nuclear chemistry , catechol oxidase , substrate (aquarium) , chromatography , organic chemistry , enzyme , botany , waste management , antioxidant , biology , peroxidase , ecology , engineering
Cracking coal-forming organic compounds during the gasification process produces liquid waste containing phenolic compounds that require special handling based on their toxicity. As one of the components, there is liquid waste resulting from the coal gasification process. The purpose of this research was to study the activity of polyphenol oxidase (PPO) on phenol after the addition of Cu 2+ to purple eggplant ( Solanum melongena L.) extract and its potential to work more effectively in phenol biodegradation for coal wastewater containing phenol. Enzyme activity and phenol determination were carried out spectrophotometrically. The results showed PPO activity of 25.90-38.10 U/mL; 4.0 mM phenol and the activity of PPO-Cu 2+ was 21.58-46.32 U/mL; 2-10 mM CuSO 4 ; 2.0-4.0 mM phenol. Based on Michaelis Menten’s graph, the initial rate of PPO-Cu 2+ was 0.015 mM/min and the initial rate of PPO was 0.15 mM/min using 2 mM phenol as a substrate. Lineweaver-Burk’s graph shows the K M of PPO-Cu 2+ = 6.92 mM, which is lower than K M of PPO = 13.05 mM. Its means that the phenol response has a higher affinity for PPO-Cu 2+ than PPO. The application of PPO-Cu 2+ in purple eggplant extract works effectively as much as 46.7% for artificial coal liquid waste containing phenol.