Open AccessThe impact of copper oxide and silver nanoparticles on woody plants obtained by in vitro method
Author(s) -
О. А. Федорова,
T.A. Grodetskaya,
N. V. Evtushenko,
Петр Евлаков,
Alexander Gusev,
Olga V. Zakharova
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/875/1/012048
Subject(s) - explant culture , nanoparticle , shoot , botany , silver nanoparticle , regeneration (biology) , micropropagation , copper , in vitro , microorganism , biology , horticulture , chemistry , materials science , nanotechnology , bacteria , biochemistry , microbiology and biotechnology , genetics , organic chemistry
The article substantiates the necessity of studying behaviour of copper oxide and silver nanoparticles on forest cultures (downy birch 29-58 ( Betula pubescens Ehrh.) and poplar ‘Pyramidal-osokoreviy Kamyshinsky’ ( Poplus pyramidalis Roz. x Poplus nigra L.) in in vitro as well as in ‘soil-plant-microbiota’ to ensure stability of forest cultures in forest regeneration. The impact of nanoparticles on shoot regeneration and propagation processes was evaluated by introducing nanoparticles into Woody Plant medium. Differences in the influence of nanoparticles on the life processes of plants depending on their concentration and the stage of clonal micropropagation have been established. The results are demonstrated by a 15-25% reduction in the frequency of infection of poplar and birch explants as well as by an increase in their regenerating potential at the stage of introduction in tissue culture. When the nanoparticle solution is used in the soil substrate, a decrease in the number of diseased plants and an increase in their survival rate of 30% can be observed. The inhibitory effect of silver nanoparticles on some ecological and trophic groups of microorganisms has been established. These results can be used in the application of CuO and Ag nanoparticles in the biotechnology of clonal micropropagation of forest crops.