Open AccessIdentification of Vibrio spp. causing vibriosis in spiny lobsters (Panulirus homarus L.) in Bengkulu marine temporary shelter ponds
Author(s) -
Risky Hadi Wibowo,
Sipriyadi Sipriyadi,
W Darwis,
Novi Susianti,
S P Yudha,
Nadya Rosianti
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/869/1/012019
Subject(s) - hepatopancreas , biology , vibrio , vibrio alginolyticus , vibrio anguillarum , fishery , spiny lobster , prawn , microbiology and biotechnology , zoology , bacteria , crustacean , ecology , genetics
Spiny lobster ( Panulirus homarus ) is one of the export commodities of the Indonesian fisheries subsector and an important component for shrimp fisheries in Indonesia. In the development of lobster cultivation, there are several obstacles, the presence of vibriosis infection caused by the pathogenic Vibrio bacteria. This study aimed to identify Vibrio spp. bacteria in spiny lobsters ( P. homarus ) reared in the marine cultivation ponds, Bengkulu, Indonesia. Clinical symptoms of lobsters infected with vibriosis are red spots on the uropod, pleopod, and abdominal parts. Bacterial isolation was conducted by isolated some internal organs in spiny lobsters, that are, gills, stomachs, haemolymph, and hepatopancreas. The result showed there are 5 isolates of Vibrio bacteria that coded by IN3, ST2, HA1, HP2, and HP3. These bacteria isolates were identified through their colony morphology and biochemical tests. Characterization on the Thiosulfate Citrate Bile Salt Sucrose (TCBS) medium showed that lobsters were infected with Vibrio species. Based on Bergey’s Manual of Determinative Bacteriology, Austin and Austin, the identification results showed that HA1 isolate was identified as Vibrio algynolyticus , IN3 isolate was identified as V. anguillarum , ST2 was identified as V. ordalii , HP2 in first lobster was identified as with V. algynolyticus that mostly in the hepatopancreas, and HP3 was identified as V. splendidus