Open AccessIsolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique
Author(s) -
Sri Agustina,
S Bella,
Sofyatuddin Karina,
I Irwan,
Maria Ulfah
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/869/1/012010
Subject(s) - phytochemistry , column chromatography , secondary metabolite , phytochemical , metabolite , chromatography , sea cucumber , extraction (chemistry) , maceration (sewage) , ethyl acetate , biology , chemistry , botany , traditional medicine , ecology , biochemistry , medicine , environmental science , environmental engineering , gene
Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra . The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.