Open AccessFeatures of the Processing of Cellulose-Containing Raw Materials with Aspergillus Niger Enzyme Systems to Obtain Bioethanol
Author(s) -
Olga Krieger,
Ekaterina Budenkova,
Olga Babich,
Станислав Сухих,
T V Voblikova
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/852/1/012056
Subject(s) - aspergillus niger , cellulose , cellulase , enzyme assay , chemistry , enzyme , raw material , strain (injury) , food science , biochemistry , nuclear chemistry , organic chemistry , biology , anatomy
In this work, studies were carried out to study the enzymatic activity of the native strain of Aspergillus niger F-1270 in relation to primary and secondary cellulose-containing raw materials in comparison with the cellulose activity of a commercial enzyme preparation. It was found that the native strain exhibits stable, but low cellulolytic activity, lower than when using an enzyme preparation under similar conditions. To enhance the cellulase activity of the native A. niger strain, nondirectional physicochemical mutagenesis was used. As a result of mutagenic action on A. niger F-1270 with UV irradiation or UV irradiation in combination with subsequent treatment with a chemical mutagen, conditionally genetically modified samples were obtained. It was confirmed that as the UV exposure time increases to two hours, the cellulase activity of the surviving part of the culture increases (r = 0.706; p <0.05). Ultraviolet irradiation with an intensity of 253.7 nm for 120 min increases the enzymatic activity of mutant strains by 2-4 times as compared to the native one. The highest enzymatic activity of relative secondary cellulose-containing raw materials is 1.5 U.