Open AccessImprovement of Culture Medium for Cultivation of Citrobacteria
Author(s) -
O N Dyptan,
N A Ozheredova,
M N Verevkina,
E V Svetlakova,
N A Gvozdetsky,
V V Bondarenko
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/852/1/012026
Subject(s) - agar , liquid medium , facultative , food science , liquid culture , agar plate , chemically defined medium , microorganism , composition (language) , chemistry , microbiology and biotechnology , biology , bacteria , chromatography , botany , biochemistry , art , in vitro , genetics , literature
The article presents the results of research on the cultivation of citrobacteria on culture media. In the experiment, culture media were used, both selective ones for citrobacteria–Simmons and Endo media, and universal ones for facultative anaerobes–meat-peptone agar (MPA). The composition of the studied culture media was injected with various concentrations of a TS-1 growth stimulant of microorganisms, which is a culture liquid of kombucha , with the production and manufacturing technology of which the authors worked. As a result of the studies, the authors found that the introduction of the 2% TS-1 growth stimulator into Simmons agar medium during the cultivation of citrobacteria gives an increase in the bacterial mass by 1.5 times more on the MPA medium and by 2 times more on the Endo medium in comparison to other concentrations of TS-1.