Open AccessGoats producing biosimilar human lactoferrin
Author(s) -
D. M. BOGDANOVICH,
В. Ф. Радчиков,
В. Н. Кузнецова,
E. V. Petrushko,
M. E. Spivak,
A. N. Sivko
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/848/1/012080
Subject(s) - lactoferrin , herd , transgene , biosimilar , offspring , immunogenicity , biology , ovalbumin , mastitis , somatic cell , recombinant dna , zoology , microbiology and biotechnology , food science , andrology , immunology , medicine , antibody , genetics , gene , pregnancy , immune system
Herd of goats producing biosimilar human lactoferrin has been created. The nature of transmission of the transgene through a number of generations, productivity according to target protein, as well as technological parameters of milk containing recombinant human lactoferrin (rhLF) have been studied. The frequency of transgene transmission from parents to offspring was recorded to be in the range of 42.4-52.6%. The median rhLF content in milk of F1 animals was 2.60 g/L, F6 – 2.00±0.85 g/L. In terms of physical and chemical and technological parameters, milk with rhLF meets quality standards. The somatic cells count, being the marker of mammary inflammation (mastitis), has been decreased in milk of producing goats.